NIOSH: PV2072: Rhodamine B by HPCL-UV-FL
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Official Method Name
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OSHA Method: RHODAMINE B - Partially Validated Method 2072 |
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Current Revision
| 1989 |
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Media
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VARIOUS |
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Instrumentation
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HPLC with dual detector-fluoresence and UV |
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Method Subcategory
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Organic |
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Method Source
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Citation
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NIOSH Manual of Analytical Methods (NMAM), 4th Ed., DHHS (NIOSH) Publication 94-113 (August, 1994), 1st Supplement Publication 96-135, 2nd Supplement Publication 98-119, 3rd Supplement 2003-154, Schlecht, P.C., and O'Connor, P.F., Eds. |
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Brief Method Summary
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This method uses HPLC with UV fluorescence detectors to identify rhodamine. Samples are extracted with methanol and analyzed by high performance liquid chromatography (HPLC) using ultraviolet (UV) or fluorescence detectors. |
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Scope and Application
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This method was developed as a stopgap method. This method has been partially evaluated and is presented for information and trial use only. It could be used for water samples with proper sample collection and preparation. |
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Applicable Concentration Range
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0.013 ng per injection with a fluorescence detector and 0.42 ng per injection with a UV detector |
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Interferences
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May have interference by non-target compounds. |
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Quality Control Requirements
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Further research is needed to develop and standardize the procedures for environmental sample types. |
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Sample Handling
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Maximum Holding Time
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Relative Cost
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Less than $50 |
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Sample Preparation Methods
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