Abraxis: 520050: Anatoxin-a by RBA, Microtiter Plate
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Official Method Name
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Abraxis Anatoxin-a RBA Kit (96T) PN 520050 |
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Current Revision
| Anatoxin-a RBA Microplate Method |
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Media
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WATER |
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Instrumentation
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Immunoassay |
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Method Subcategory
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Organic |
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Method Source
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Citation
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Brief Method Summary
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The test is a receptor-binding assay based on the affinityof Anatoxin-a for nicotinic acetylcholine receptors (nAChRs). Anatoxin-a, when present in a sample, will compete with the biotinylated alpha-bungarotoxin for the acetylcholine binding sites of nAChRs, from purified Torpedoelectrocyte membranes, which were coated on the microtiter plate. After a washing step, a streptavidin-HRP solution is added to allow for the colorimetric detection of the biotinylated alpha-bungarotoxin bound on the receptor. After a second washing step, the substrate solution is added and a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Anatoxin-a present in the sample. The color reaction is stopped after a specified time and the color (yellow) is evaluated using a microplate photometer. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run. |
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Scope and Application
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Applicable Concentration Range
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10 to 500; 0.4 to 20 (ES) |
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Interferences
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Quality Control Requirements
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Sample Handling
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Maximum Holding Time
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Approximately one year at 4 degrees |
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Relative Cost
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Less than $50 |
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Sample Preparation Methods
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