EPA-OW: NRSA Fecal 2009 (Boat): Fecal Indicator sampling in large non-wadeable rivers
|
Official Method Name
|
Section 5.7: Non-Wadeable Rivers-Fecal Indicator (Enterococci) (of National Rivers and Streams Assessment: Field Operations Manual (2009)) |
|---|---|
|
Current Revision
| 2009 |
|
Media
|
WATER (Waterbody type - Non-wadeable stream) |
|
Instrumentation
|
Field Equipment |
|
Method Subcategory
|
Microbiological |
|
Method Source
|
|
|
Citation
|
|
|
Protocol
|
EPA-841-B-07-009 - National Rivers and Streams Assessment: Field Operations Manual (2009) |
|
Brief Method Summary
|
Collect a fecal indicator sample at the last transect after all other sampling is completed. Samples must be filtered and the filters must be frozen within 6 hours of collection. Use a pre-sterilized, 250 ml bottle and collect the sample approximately 1 m off the bank at about 1 meter below the water surface. Add the sodium thiosulfate tablet, cap, and shake bottle 25 times. Following collection, place the sample in a cooler, chill for at least 15 minutes, and maintain on ice prior to filtration of four 50mL volumes. |
|
Scope and Application
|
Methods described in this document are to be used specifically in work relating to the National Rivers and Streams Assessment. This document contains proceedures for how to collect a fecal indicator sample. |
|
Applicable Concentration Range
|
|
|
Interferences
|
Follow the guidelines for choosing collection site. |
|
Quality Control Requirements
|
Collect sample in a pre-sterilized, 250 ml sample bottle |
|
Sample Handling
|
Approach the sampling location slowly from downstream or downwind. Collect sample approximately 1 m from bank and 0.3 m (1 foot) deep. Add sodium thiosulfate tablet to preserve sample. |
|
Maximum Holding Time
|
|
|
Relative Cost
|
Unknown |
|
Sample Preparation Methods
|
Samples must be filtered and frozen on dry ice within 6 hours of collection. |
