EPA-OW: NRSA Fecal 2009 (Wade): Fecal Indicator sampling in small wadeable streams
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Official Method Name
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Section 6.6: Wadeable streams-Fecal Indicator (Enterococci) (of National Rivers and Streams Assessment: Field Operations Manual (2009)) |
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Current Revision
| 2009 |
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Media
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WATER (Waterbody type - Wadeable stream) |
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Instrumentation
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Field Equipment |
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Method Subcategory
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Microbiological |
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Method Source
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Citation
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Protocol
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EPA-841-B-07-009 - National Rivers and Streams Assessment: Field Operations Manual (2009) |
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Brief Method Summary
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Collect a fecal indicator sample at the last transect after all other sampling is completed. Samples must be filtered and the filters must be frozen within 6 hours of collection. Use a pre-sterilized, 250 ml bottle and collect the sample approximately 1 m off the bank at about 0.3 meter (12 inches) below the water surface. Add the sodium thiosulfate tablet, cap, and shake bottle 25 times. Following collection, place the sample in a cooler, chill for at least 15 minutes, and maintain on ice prior to filtration of four 50mL volumes. |
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Scope and Application
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Methods described in this document are to be used specifically in work relating to the National Rivers and Streams Assessment. This document contains proceedures for how to collect a fecal indicator sample. |
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Applicable Concentration Range
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Interferences
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Follow the guidelines for choosing collection site. |
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Quality Control Requirements
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Collect sample in a pre-sterilized, 250 ml sample bottle |
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Sample Handling
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Approach the sampling location slowly from downstream or downwind. Collect sample approximately 1 m from bank and 0.3 m (1 foot) deep. Add sodium thiosulfate tablet to preserve sample. |
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Maximum Holding Time
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Relative Cost
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Unknown |
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Sample Preparation Methods
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Samples must be filtered and frozen on dry ice within 6 hours of collection. |
