Beacon: 20-0173: Saxitoxin in water by immunoassay, Microtiter Plate
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Official Method Name
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Saxitoxin Plate Kit |
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Current Revision
| 2014 |
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Media
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WATER |
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Instrumentation
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Immunoassay |
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Method Subcategory
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Biotoxin |
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Method Source
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Citation
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Brief Method Summary
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The Saxitoxin HRP conjugate, sample and calibrators are pipetted into the test wells followed by Saxitoxin antibody into the test wells to initiate the reaction. During the 30 minute incubation period, Saxitoxin from the sample and Saxitoxin HRP conjugate compete for binding to Saxitoxin antibody. The Saxitoxin antibody is captured on the walls of the test well. Following this 30 minute incubation, the contents of the well are removed and the wells are washed to remove any unbound Saxitoxin, Saxitoxin HRP conjugate and free Saxitoxin antibody. After wash, a clear substrate is then added to the wells and any bound enzyme conjugate causes the conversion to a blue color. Following a 30 minute incubation, the reaction is stopped and the amount of color in each well is read. The color of the unknown samples is compared to the color of the calibrators and the Saxitoxin concentration of the samples is derived. |
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Scope and Application
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This method determines saxitoxin in water. |
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Applicable Concentration Range
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0.02 - 0.4 |
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Interferences
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Quality Control Requirements
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Sample Handling
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Water sample is mixed with sample diluent and stored at 4 degree Celsius. |
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Maximum Holding Time
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Relative Cost
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Unknown |
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Sample Preparation Methods
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1.Dilute water sample with the provided Freshwater Diluent (900 μL of water sample + 100 μL of Freshwater Diluent). 2.Mix thoroughly. 3.Filtering samples with visible particulates is recommended. If filtration is needed, use Whatman #1 filter paper or a syringe filter (such as SLGS 025 NB, SLAA 025 NB or SLAA 025 NK from Millipore) after dilution. 4.Follow the assay procedure in the Saxitoxin plate kit instructional booklet. 5.Apply dilution factor of 1.1 to the assay result to calculate the saxitoxin concentration in the original water sample. |
