Beacon: 20-0149: Cylindrospermopsin in water by immunoassay, Microtiter Plate
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Official Method Name
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Cylindrospermopsin Plate Kit |
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Current Revision
| 2011 |
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Media
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WATER |
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Instrumentation
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Immunoassay |
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Method Subcategory
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Biotoxin |
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Method Source
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Citation
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Brief Method Summary
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Cylindrospermopsin plate kit is a competitive enzyme-labeled immunoassay. The Cylindrospermopsin -HRP enzyme conjugate is pipetted into test wells followed by calibrators or sample extracts. Cylindrospermopsin Antibody Solution is then pipetted into the test wells to initiate the reaction. During the 45-minute incubation period, Cylindrospermopsin from the sample and Cylindrospermopsin HRP conjugate compete for binding to Cylindrospermopsin antibody. The Cylindrospermopsin antibody is captured on the walls of the test well. Following this 45-minute incubation, the contents of the well are removed and the wells are washed to remove any unbound Cylindrospermopsin, Cylindrospermopsin HRP conjugate and free Cylindrospermopsin antibody. After this wash step, a clear substrate is then added to the wells and any bound enzyme conjugate causes the conversion to a blue color. Following a 45-minute incubation, the reaction is stopped and the amount of color in each well is read. The color of the unknown samples is compared to the color of the calibrators and the Cylindrospermopsin concentration of the samples is derived. |
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Scope and Application
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This method determines Cylindrospermopsin in water. |
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Applicable Concentration Range
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0.1 - 2.0 |
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Interferences
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Cross reactivity: MICROCYSTIN-LR (<1%) and NODULARIN (<1%). |
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Quality Control Requirements
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Sample Handling
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Maximum Holding Time
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Relative Cost
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Unknown |
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Sample Preparation Methods
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