EPA-OW: EPA RBP (Inverts MHAB): Benthic Macroinvertebrate RBP, multiple habitat, D-frame dip net
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Official Method Name
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Benthic Macroinvertebrate Protocols: 7.2, Multiple Habitat Approach: D-frame Dip Net |
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Current Revision
| Second Edition, 1999 |
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Media
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WATER (Waterbody type - Wadeable stream) |
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Instrumentation
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Invertebrate Net (w/ handle) |
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Method Subcategory
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Population/Community |
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Method Source
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Citation
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Protocol
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EPA RBP Protocol - Rapid Bioassessment Protocols for Use in Streams and Wadeable Rivers: Periphyton, Benthic Macroinvertebrates, and Fish - Second Edition |
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Brief Method Summary
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A 100m reach representative of the characteristics of the stream should be selected that is at least 100m from any road, bridge crossing, or major tributary. Prior to sampling, the physical/chemical field sheet should be completed and a map should be drawn showing in-stream attributes and structures. Sampling begins at the downstream portion of the reach and proceeds upstream. Different types of habitat are to be sampled in approximate proportion to their representation of surface area of the total macroinvertebrate habitat in the reach. For example, if snags comprise 50% of the habitat in a reach and riffles comprise 20%, then 10 jabs should be taken in snag material and 4 jabs should be take in riffle areas. The remainder of the jabs (6) would be taken in any remaining habitat type. Habitat types contributing less than 5% of the stable habitat in the stream reach should not be sampled. The jabs or kicks collected from the multiple habitats will be composited to obtain a single homogeneous sample. |
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Scope and Application
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This protocol is appropriate for the sampling of wadeable streams and rivers. Programmatic applications of the biological data generated using these methods include, CWA Section 305(b) water quality assesments, CWA Section 319 nonpoint source assessments, watershed protection, CWA Section 303(d) TMDL development, CWA Section 402 NPDES permits and individual control strategies, ecological risk assessment, and development of USEPA water quality criteria and standards |
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Applicable Concentration Range
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Interferences
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Every 3 jabs, more often if necessary, wash the collected material by running clean stream water through the net two to three times. |
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Quality Control Requirements
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Proper sample labeling; Net, pan, etc. rinsed thoroughly between samples; Replicate (1 duplicate sample) collected at 10% of sites |
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Sample Handling
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Rinse large debris and place any organisms found into sample container; place all small debris directly into sample container. Preserve in 95% ethanol sufficient to cover the sample. Place a label indicating the sample identification code or lot number, date, stream name, sampling location, and collector name into the sample container. The outside of the container should include the same information and the words "preservative, 95% ethanol". If more than one container is needed for a sample, each container label should contain all of the information for the sample and should be numbered (e.g., 1 of 2, 2 of 2, etc) |
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Maximum Holding Time
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Indefinite with proper preservation. |
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Relative Cost
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Greater than 6 hrs. per site/sample |
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Sample Preparation Methods
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95% ethanol |
