Beacon: 20-0099: Aflatoxin in grains by immunoassay, Coated Tube
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Official Method Name
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Aflatoxin Tube Kit |
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Current Revision
| 2013 |
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Media
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AGRICULTURAL PRODUCTS |
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Instrumentation
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Immunoassay |
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Method Subcategory
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Biotoxin |
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Method Source
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Citation
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Brief Method Summary
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Aflatoxin is extracted from a ground sample by shaking with methanol/water. The extract is diluted with water and filtered and then filtered and then is tested in the immunoassay. Aflatoxin-HRP enzyme conjugate is pipetted into the test tubes followed by calibrators or sample extracts. Aflatoxin antibody is then pipetted into the test tubes to initiate the reaction. During the 10 minute incubation period, aflatoxin from the sample and aflatoxin-HRP enzyme conjugate compete for binding to the aflatoxin antibody which, in turn, binds to the test tube. Following this 10 minute incubation, the contents of the tube are removed and the tubes are washed to remove any unbound toxin or enzyme-labeled toxin. A clear substrate is then added to the tubes and any bound enzyme-toxin conjugate causes the conversion to a blue color. Following a 10 minute incubation, the reaction is stopped and amount of color in each tube is read. The color of unknown samples is compared to the color of the calibrators and the Aflatoxin concentration of the samples is derived. |
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Scope and Application
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This method determines aflatoxin in grains. |
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Applicable Concentration Range
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2 - 100 |
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Interferences
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Quality Control Requirements
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Sample Handling
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Maximum Holding Time
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Relative Cost
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Unknown |
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Sample Preparation Methods
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