Beacon: 20-0230: Aflatoxin in grains by immunoassay, Coated Tube with less usage
|
Official Method Name
|
Quan-Tox Aflatoxin Tube Kit |
|---|---|
|
Current Revision
| 2014 |
|
Media
|
AGRICULTURAL PRODUCTS |
|
Instrumentation
|
Immunoassay |
|
Method Subcategory
|
Biotoxin |
|
Method Source
|
|
|
Citation
|
|
|
Brief Method Summary
|
Aflatoxin is extracted from ground samples by shaking with methanol/water. The extract is diluted with water, filtered and tested in the immunoassay. Aflatoxin-HRP enzyme conjugate is pipetted into the test tubes followed by Zero Calibrator or sample extracts. Aflatoxin antibody is then pipetted into the test tubes to initiate the reaction. During the 10 minute incubation period, aflatoxin from the sample and aflatoxin-HRP enzyme conjugate competes for binding to aflatoxin antibody which, in turn, binds to the test tube. Following this 10 minute incubation, the contents of the tube are removed and the tubes are washed to remove any unbound aflatoxin or aflatoxin-HRP conjugate. A clear substrate is then added to the tubes and any bound aflatoxin-HRP conjugate causes the conversion to a blue color. Following a 10 minute incubation, the reaction is stopped and amount of color in each tube is read. The color intensities of sample and Zero Calibrator are measured at 450 nm. The ratio of sample to Zero Calibrator (%Bo) is calculated, and the aflatoxin concentration of the sample is derived. |
|
Scope and Application
|
This method determines aflatoxin in grains. |
|
Applicable Concentration Range
|
2 - 100 |
|
Interferences
|
|
|
Quality Control Requirements
|
|
|
Sample Handling
|
|
|
Maximum Holding Time
|
|
|
Relative Cost
|
Unknown |
|
Sample Preparation Methods
|
