Beacon: 20-0119: Zearalenone in grains by immunoassay, Coated Tube
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Official Method Name
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Zearalenone Tube Kit |
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Current Revision
| 2014 |
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Media
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AGRICULTURAL PRODUCTS |
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Instrumentation
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Immunoassay |
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Method Subcategory
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Biotoxin |
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Method Source
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Citation
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Brief Method Summary
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Zearalenone is extracted from a ground sample by shaking with methanol/water. The extract is filtered and diluted. The extract is then tested in the immunoassay. Zearalenone-HRP enzyme conjugate is pipetted into the test tubes followed by calibrators or sample extracts. The Zearalenone antibody is then pipetted into the test tubes to initiate the reaction. During the 10 minute incubation period, Zearalenone from the sample and Zearalenone-HRP enzyme conjugate compete for binding to Zearalenone antibody which is bound to the test tube. Following this 10 minute incubation, the contents of the tube are removed and the tubes are washed to remove any unbound enzyme-labeled toxin. A clear substrate is then added to the tubes and any bound enzyme-toxin conjugate causes the conversion to a blue color. Following a 10 minute incubation, the reaction is stopped and the amount of color in each tube is read. The color of unknown samples is compared to the color of the calibrators and the Zearalenone concentration of the samples is derived. |
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Scope and Application
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This method determines zearalenone in grains. |
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Applicable Concentration Range
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10 - 100 |
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Interferences
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Quality Control Requirements
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Sample Handling
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Maximum Holding Time
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Relative Cost
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Unknown |
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Sample Preparation Methods
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