Abraxis: 520060: Anatoxin-a by Immunoassay, Microtiter Plate
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Official Method Name
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Abraxis Anatoxin-a ELISA (96T) PN 520060 |
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Current Revision
| Anatoxin-a ELISA Microplate Method |
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Media
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WATER |
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Instrumentation
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Immunoassay |
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Method Subcategory
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Organic |
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Method Source
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Citation
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Brief Method Summary
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The test is a direct competitive ELISA based on the recognition of Anatoxin-a by a monoclonal antibody. Anatoxin-a, when present in a sample, and an Anatoxin-a-enzyme conjugate compete for the binding sites of mouse anti-Anatoxin-a antibodies in solution. The Anatoxin-a antibodies are then bound by a second antibody (anti-mouse) immobilized on the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Anatoxin-a present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run. |
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Scope and Application
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This method determines anatoxin-a in water samples. |
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Applicable Concentration Range
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0.15 - 5.0 |
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Interferences
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Quality Control Requirements
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Sample Handling
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Maximum Holding Time
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Approximately one year at 4 degrees |
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Relative Cost
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Less than $50 |
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Sample Preparation Methods
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