USGS: Marion Invert 1999-2001: Marion County Invertebrate Collection Protocol for Wadeable Streams, Surber sampler, 1999-2001
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Official Method Name
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Benthic Macroinvertebrates, Biological Assessment of Streams in the Indianapolis Metropolitan Area, Indiana, 1999–2001 |
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Current Revision
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Media
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WATER |
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Instrumentation
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Invertebrate Fixed-Area Sampler |
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Method Subcategory
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Population/Community |
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Method Source
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Citation
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Protocol
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USGS WRIR 03–4331 - Biological Assessment of Streams in the Indianapolis Metropolitan Area, Indiana, 1999–2001 |
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Brief Method Summary
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Benthic-invertebrate samples were collected by use of Surber samplers that had a 0.0929 sq m sample grid and a collection-net bag-mesh opening of 210 um. This mesh size has been recommended for collecting benthic invertebrates (Britton and Greeson, 1988). Surber samplers were placed on the streambed, and all the large surface materials within the sample grid were removed and placed in buckets for subsequent scrubbing. The area within the sample grid was scrubbed to remove benthic invertebrates attached to the substrate or embedded in the upper inch of the streambed sediments. Benthic invertebrates were preserved in 10-percent formalin and shipped to a contract laboratory for identification.
Benthic invertebrates were identified to the lowest tax-onomic group possible—often genus and species. The total number of organisms, number of distinct taxa, and EPT Index scores were summarized for each of the three samples collected at each site. The results of the three samples were combined to determine the EPT, HBI, and ICI scores for the site. The total number of distinct taxa for each sampling site was calculated by resolving ambiguous taxa, then summing the unique taxa for each sample. For the purpose of this report, ambiguous taxa are those not identified to species; they were counted as a distinct taxon only if there were no reported individuals from the next-highest common taxonomic level. Possible reasons for inability to classify to species may be that the organism was damaged or only partially present in the sample, or that the organism was at a life stage that could not be classified to a lower taxon. |
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Scope and Application
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Applicable Concentration Range
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Interferences
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Quality Control Requirements
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Sample Handling
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Maximum Holding Time
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Relative Cost
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Unknown |
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Sample Preparation Methods
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