EPA: 8318 A: N-methylcarbamates in soil, water, and waste matrices by high performance liquid chromatography (HPLC)

Summary
Analytes
Revision
Data and Sites

Official Method Name

EPA Method 8318A: N-methylcarbamates by high performance liquid chromatography (HPLC)

Current Revision

Revision 1, February 2007

Media

VARIOUS

Instrumentation

High Performance Chromatography with Post Column Derivitization and Fluorescence Detection

Method Subcategory

Organic

Method Source

EPA

Citation

SW-846 Online: Test Methods for Evaluating Solid Waste, Physical/Chemical Methods

Brief Method Summary

N-methylcarbamates are extracted from samples using various 3500 series extraction methods, or other methods that are appropriate for the sample matrix. Extracts may be subjected to an optional cleanup procedure designed to remove interferences. During cleanup, the solvent is exchanged to methanol for analysis. Extracts are analyzed by HPLC, on a C-18 reversed-phase column. After separation, the target analytes are hydrolyzed and derivatized post-column, then quantitated.

Scope and Application

This method covers the determination of N-methylcarbamates in soil, water, and waste matrices

Applicable Concentration Range

Not given

Interferences

(A) Solvents, reagents, glassware, and other sample processing hardware contamination: Analyze method blanks, distill reagents and solvents in all-glass systems if necessary. (B) Fluorescent compounds, primarily alkyl amines and compounds which yield primary alkyl amines upon base hydrolysis: No remedy. (C) Coeluting compounds that are fluorescence quenchers may result in negative interferences: No remedy. (D) Also refer to Methods 3500 and 8000.

Quality Control Requirements

Mixed QC standard, method blank, laboratory control sample (LCS), (optional) surrogates, matrix spike, and matrix spike duplicate, solvent blank, clean refence matrix, calibration verification standards,

Sample Handling

Preserve immediately (acidify to pH 4-5 with 0.1 N chloroacetic acid). Store samples at 6 ˚C out of direct sunlight from the time of collection through analysis.

Maximum Holding Time

7 days for extraction after collection, 40 days for analysis after extraction

Relative Cost

Sample Preparation Methods

Various 3500 series extraction methods, other methods, some of which given here.

This method has 17 analytes associated with it.

Analyte	Detection Level	Bias	Precision	Spiking Level
3-Hydroxycarbofuran(16655-82-6) 3,7-Benzofurandiol, 2,3-dihydro-2,2-dimethyl-, 3-Hydroxycarbofuran	N/A	70% Rec (SL)	3.70 % RSD (SL)	300.00 ug/L
Aldicarb(116-06-3) 2-Methyl-2-(methylthio)propanal, O-((methylamino)carbonyl)oxime Aldicarb Carbanolate Temik UC-21149	N/A	75% Rec (SL)	6.00 % RSD (SL)	300.00 ug/L
Aldicarb sulfone(1646-88-4) Aldicarb sulfone Aldicarb sulfone, wh Aldoxycarb Aldoxycarb (ANSI) Standak Sulfocarb Temik sulfone	N/A	75% Rec (SL)	3.20 % RSD (SL)	300.00 ug/L
Bendiocarb(22781-23-3) Bendiocarb	N/A	N/A	N/A
Carbaryl(63-25-2) 1-Naphthyl methylcarbamate 1-Naphthyl-N-methylcarbamate Carbamic acid, methyl-, 1-naphthyl ester Carbaril Carbaryl Carbaryl (ANSI) Carbaryl [1-naphthalenol, methylcarbamate] Sevimol Sevin	N/A	81% Rec (SL)	3.50 % RSD (SL)	300.00 ug/L
Carbofuran(1563-66-2) 2,3-Dihydro-2,2-dimethyl-7-benzofuranyl methylcarbamate Carbofuran Carbofuran (ANSI) Dihydro-2,2-dimethyl-7-benzofuranyl methylcarbamate,2,3- Euradan Furadan	N/A	80% Rec (SL)	3.90 % RSD (SL)	300.00 ug/L
Dioxacarb(6988-21-2) Dioxacarb	N/A	80% Rec (SL)	3.50 % RSD (SL)	300.00 ug/L
Formetanate hydrochloride(23422-53-9) Formetanate hydrochloride	N/A	N/A	N/A
Methiocarb(2032-65-7) 3,5-Dimethyl-4-(methylthio)phenol methylcarbamate 4-(Methylthio)-3,5-xylyl methylcarbamate Carbamic acid, methyl-, 4-(methylthio)-3,5-xyl Carbamic acid, methyl-, 4-(methylthio)-3,5-xylyl ester Mercaptodimethur Mesurol Methiocarb Phenol, 3,5-dimethyl-4-(methylthio)-, methylca Phenol, 3,5-dimethyl-4-(methylthio)-, methylcarbamate	N/A	77% Rec (SL)	3.50 % RSD (SL)	300.00 ug/L
Methomyl(16752-77-5) Acetamidic acid, thio-N-[(methyl-carbamoyl)oxy Acetamidic acid, thio-N-[(methyl-carbamoyl)oxy]-, ... Dupont 1179 Insecticide 1179 Lannate Methomyl S-methyl-n-((methylcarbamoyl)oxy)thioacetimidate	N/A	81% Rec (SL)	3.40 % RSD (SL)	300.00 ug/L
Metolcarb(1129-41-5) Metolcarb	N/A	N/A	N/A
Mexacarbate(315-18-4) 4-(Dimethylamino)-3,5-dimethylphenyl methylcarbamate Carbamic acid, methyl-, 4-dimethylamino-3,5-xy Carbamic acid, methyl-, 4-dimethylamino-3,5-xylyl ester Dimethylamino-3,5-xylyl methylcarbamate,4- Mexacarbate Mexacarbate (ANSI) Mexacarbole Phenol, 4-(di-methylamino)-3,5-dimethyl, methy Zectran	N/A	N/A	N/A
Oxamyl(23135-22-0) 2-o-Methylcarbamoyl monoxime DPX 1410 Oxamyl Thioxamyl Vydate Vydate L	N/A	N/A	N/A
Promecarb(2631-37-0) Promecarb	N/A	76% Rec (SL)	4.10 % RSD (SL)	300.00 ug/L
Propoxur(114-26-1) 2-Isopropoxyphenyl-n-methylcarbamate Aprocarb Baygon Blattenex Isocarb N-methyl-2-isopropoxyphenylcarbamate Phenol, 2-(1-methylethoxy)-, methylcarbamate Propoxur Propoxur [phenol, 2-(1-methylethoxy)-, methylcarbamate] Sendran, Suncide Unden o-Isopropoxyphenyl methylcarbamate	N/A	77% Rec (SL)	4.60 % RSD (SL)	300.00 ug/L
Thiodicarb(59669-26-0) Thiodicarb	N/A	N/A	N/A
m-Cumenyl methylcarbamate(64-00-6) m-Cumenyl methylcarbamate	N/A	N/A	N/A

Precision Descriptor Notes:	Single operator, spiked reagent water, 300 ug/L of each compound, n = 10. Performance data and related information are provided in SW-846 methods only as examples and guidance. The data do not represent required performance criteria for users of the methods. These performance data are not intended to be and must not be used as absolute QC acceptance criteria for purposes of laboratory accreditation.
Detection Level Note:	Refer to Method 8000 for specific determinative method QC procedures. Refer to Method 3500 for QC procedures to ensure the proper operation of the various sample preparation techniques that can be applied to this method, which contains its own extraction procedures. If an extract cleanup procedure is performed, refer to Method 3600 for the appropriate QC procedures. Any more specific QC procedures provided in this method will supersede those noted in Methods 8000, 3500, 3600, or 5000.

Precision Descriptor Notes:

Single operator, spiked reagent water, 300 ug/L of each compound, n = 10. Performance data and related information are provided in SW-846 methods only as examples and guidance. The data do not represent required performance criteria for users of the methods. These performance data are not intended to be and must not be used as absolute QC acceptance criteria for purposes of laboratory accreditation.

Detection Level Note:

Refer to Method 8000 for specific determinative method QC procedures. Refer to Method 3500 for QC procedures to ensure the proper operation of the various sample preparation techniques that can be applied to this method, which contains its own extraction procedures. If an extract cleanup procedure is performed, refer to Method 3600 for the appropriate QC procedures. Any more specific QC procedures provided in this method will supersede those noted in Methods 8000, 3500, 3600, or 5000.

Revision	PDF/Link
Revision 1, February 2007

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EPA: 8318 A: N-methylcarbamates in soil, water, and waste matrices by high performance liquid chromatography (HPLC)

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