Syngenta: AG-625: Atrazine by Immunoassay

Summary
Analytes
Revision
Data and Sites

Official Method Name

Atrazine in Drinking Water by Immunoassay

Current Revision

Feb-01

Media

WATER

Instrumentation

Immunoassay

Method Subcategory

Organic

Method Source

Syngenta

Citation

Atrazine in Drinking Water by Immunoassay

Brief Method Summary

Atrazine is detected using a colorimetric immunoassay procedure. A 0.5 mL sample is to a polystyrene culture tube pre-coated with atrazine antibodies. Atrazine in the sample competes with an enzyme conjugate solution for the finite number of antibody binding sites. The tube is washed and the enzyme substrate is added. The substrate is enzymatically converted from a colorless to a blue solution until terminated by acidification. The concentration of atrazine is determined by measuring the absorbance of the solution with a photometer.

Scope and Application

This method determines atrazine in raw and finished drinking water.

Applicable Concentration Range

0.05-5.0

Interferences

(A) Cross-reactivity: Propazine and prometon produce false positive responses for atrazine.

(B) Temperature: Temperature should be kept constant near 22 degrees Celsius for accurate measurements. Avoid drafts and air vents.

Quality Control Requirements

The quality control elements in this method include a method detection limit (MDL) study, an initial precision and recovery (IPR) study, matrix spike/duplicate (MS/MSDs) analyses, and method blank analyses. Also, a full calibration curve is run with each method sample batch.

Sample Handling

Collect 5 mL of sample in a glass container and refrigerate at approximately 4^oC. For compliance monitoring samples should be held no longer than 14 days. However, Syngenta performed a study that indicates that the holding time may be extended to two years.

Maximum Holding Time

14 days (for compliance monitoring)
2 years otherwise (see Sample Handling field).

Relative Cost

Less than $50

Sample Preparation Methods

This method has 2 analytes associated with it.

Analyte	Detection Level	Bias	Precision	Pct False Positive	Pct False Negative	Spiking Level
Atrazine(1912-24-9) 1,3,5-Triazine-2,4-diamine, 6-chloro-N-ethyl-N'-(1- ... 2-Chloro-4-(ethylamino)-6-(isopropylamino)-S-triazine 2-Chloro-4-(ethylamino)-6-(isopropylamino)-s-t 2-Chloro-4-ethylamino-6-isopropylamine-sym-triazine AAtrex Atratol 8P Atrazine Atrazine (ANSI) Bicep	0.050 ppb	109% Rec (ML)	11.30 % RSD (ML)			0.50 ppb
Herbicides(E-12839) Herbicide formulations Herbicides	N/A	N/A	N/A

Precision Descriptor Notes:	Precision and accuracy values were calculated using data from nineteen participant laboratories. Each laboratory performed four replicate analyses of a fortified reagent water sample to generate the raw data. Syngenta Corporation prepared a full validation study report (separate from the method; the source of precision and accuracy values) that characterizes performance in a variety of water matrices.
Detection Level Note:	The MDL represents the pooled MDLs of nineteen laboratories. MDLs were determined according to 40 CFR part 136, Appendix B at each laboratory.

Precision Descriptor Notes:

Precision and accuracy values were calculated using data from nineteen participant laboratories. Each laboratory performed four replicate analyses of a fortified reagent water sample to generate the raw data. Syngenta Corporation prepared a full validation study report (separate from the method; the source of precision and accuracy values) that characterizes performance in a variety of water matrices.

Detection Level Note:

The MDL represents the pooled MDLs of nineteen laboratories. MDLs were determined according to 40 CFR part 136, Appendix B at each laboratory.

Revision	PDF/Link
Feb-01

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Syngenta: AG-625: Atrazine by Immunoassay

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