EPA-EAD: 610: Polynuclear Aromatic Hydrocarbons by GC

Summary
Analytes
Revision
Data and Sites

Official Method Name

Polynuclear Aromatic Hydrocarbons

Current Revision

40 CFR Part 136, Appendix A (Current Edition)

Media

WATER

Instrumentation

High Performance Liquid Chromatography

Method Subcategory

Organic

Method Source

EPA-EAD

Citation

EPA Method Guidance CD-ROM (includes MCAWW Methods, and most current EPA Methods)

Brief Method Summary

A measured volume of sample, approximately 1L, is extracted with methylene chloride using a separatory funnel. The methylene chloride extract is dried and concentrated. The extract is then sepearated by HPLC or GC. Ultraviloet (UV) and fluoresence detectors are used with HPLC to identify and measure PAHs. A flame ionization detector is used with GC.

Scope and Application

This method allows for determination of polynuclear aromatic hydrocarbons (PAHs) in municipal and industrial discharges as provided under 40 CFR 136.1.

Applicable Concentration Range

DL - * (* Upper detection limit is defined as the highest calibration point, as determined by analyst).

Interferences

(1) Method interferences caused by contaminants in solvents, reagents, glassware, and other sample processing hardware that lead to discrete artifacts and/or elevated baselines in the chromatograms. (2) Matrix interferences caused by contaminants co-extracted from the sample.

Quality Control Requirements

reagent blanks (RB), quality control samples (QCS)

Sample Handling

Collect samples in glass containers. If samples contain residual chlorine, add 80 mg of sodium thiosulfate per liter of sample. All samples must be refrigerated.

Maximum Holding Time

Relative Cost

$201 to $400

Sample Preparation Methods

This method has 18 analytes associated with it.

Analyte	Detection Level	Bias	Precision	Spiking Level
Acenaphthene(83-32-9) 1,2-Dihydroacenaphthylene 1,8-Ethylenenaphthalene Acenaphthene Acenaphthene,1,2-dihydro- Acenaphthylene, 1,2-dihydro- Periethylenenaphthalene	1.800 ug/L	55% Rec (ML)	65.00 % RSD (ML)	20.00 ug/L
Acenaphthylene(208-96-8) Acenaphthalene Acenaphthylene Cyclopenta(de)naphthalene	2.300 ug/L	60% Rec (ML)	46.00 % RSD (ML)	20.00 ug/L
Anthracene(120-12-7) Anthracene Anthracin Green oil Paranaphthalene Tetra olive n2g	0.660 ug/L	57% Rec (ML)	45.00 % RSD (ML)	20.00 ug/L
Benz[a]anthracene(56-55-3) 1,2-Benzanthracene 2,3-Benzphenanthrene Benz[a]anthracene Benzanthrene Benzo(a)anthracene Benzoanthracene Naphthanthracene Tetraphene	0.010 ug/L	73% Rec (ML)	34.00 % RSD (ML)	20.00 ug/L
Benzo(a)pyrene(50-32-8) 3,4-Benzopyrene 3,4-Benzpyrene BP BaP Benzo(a)pyrene Benzo[a]pyrene	0.020 ug/L	56% Rec (ML)	53.00 % RSD (ML)	20.00 ug/L
Benzo(b)fluoranthene(205-99-2) 2,3-Benzofluoranthene 3,4-Benzfluoranthene 3,4-Benzofluoranthene B(b)F Benz[e]acephenanthrylene Benzo(b)fluoranthene Benzo(e)fluoranthene Benzo[b]fluoranthene	0.018 ug/L	78% Rec (ML)	38.00 % RSD (ML)	20.00 ug/L
Benzo(g,h,i)perylene(191-24-2) 1,12-Benzoperylene 1,12-Benzperylene B(ghi)P Benzo(g,h,i)perylene Benzo(ghi)perylene	0.076 ug/L	46% Rec (ML)	59.00 % RSD (ML)	20.00 ug/L
Benzo(k)fluoranthene(207-08-9) 11,12-Benzofluoranthene B(k)F Benz(k)fluoranthene Benzo(k)fluoranthene	0.017 ug/L	59% Rec (ML)	69.00 % RSD (ML)	20.00 ug/L
Chrysene(218-01-9) 1,2,5,6-Dibenzonaphthalene 1,2-Benzophenanthrene 1,2-Benzphenanthrene Benz(a)phenanthrene Benzo(a)phenanthrene Chrysene	0.150 ug/L	76% Rec (ML)	65.00 % RSD (ML)	20.00 ug/L
Dibenzo(a,h)anthracene(53-70-3) 1,2:5,6-Dibenzanthracene DB(a,h)A Dibenz(a,h)anthracene Dibenzo(a,h)anthracene	0.030 ug/L	42% Rec (ML)	45.00 % RSD (ML)	20.00 ug/L
Fluoranthene(206-44-0) 1,2-Benzacenaphthene Benzo(j,k)fluorene Fluoranthene Idryl	0.210 ug/L	68% Rec (ML)	32.00 % RSD (ML)	20.00 ug/L
Fluorene(86-73-7) 2,2'-Methylenebiphenyl 9H-Fluorene Diphenylenemethane Fluorene o-Biphenylenemethane	0.210 ug/L	53% Rec (ML)	57.00 % RSD (ML)	20.00 ug/L
Indeno(1,2,3-cd)pyrene(193-39-5) 1,10-(1,2-Phenylene)pyrene 2,3-Phenylenepyrene Indeno(1,2,3-cd)pyrene o-Phenylenepyrene	0.040 ug/L	54% Rec (ML)	42.00 % RSD (ML)	20.00 ug/L
Naphthalene(91-20-3) Moth balls Naphthalene Naphthalin Naphthene Tar camphor White tar	1.800 ug/L	54% Rec (ML)	48.00 % RSD (ML)	20.00 ug/L
Organics, semivolatile(E-12884) Organics, semivolatile Semi-volatile organics Semivolatile organics	N/A	N/A	N/A
Phenanthrene(85-01-8) Phenanthrene Phenathrin	0.640 ug/L	67% Rec (ML)	45.00 % RSD (ML)	20.00 ug/L
Pyrene(129-00-0) Benzo[def]phenanthrene Pyrene Pyrene[def]phenanthrene	0.270 ug/L	68% Rec (ML)	42.00 % RSD (ML)	20.00 ug/L
Volatile organic compounds(E-12419) Organic compounds, volatile VOCs Volatile organic compounds	N/A	N/A	N/A

Precision Descriptor Notes:	The precision and accuracy values were determined using regression equations derived from a 16 laboratory validation study. The study determined analytes using reagent water, drinking water, surface water, and three industrial wastewaters with known additions at six concentrations over the range 0.1 to 425 ?g/L. Single operator precision, overall precision, and accuracy were found to be directly related to the concentration of the parameter and essentially independent of the sample matrix.
Detection Level Note:	MDLs determined per 40 CFR part 136, Appendix B.

Precision Descriptor Notes:

The precision and accuracy values were determined using regression equations derived from a 16 laboratory validation study. The study determined analytes using reagent water, drinking water, surface water, and three industrial wastewaters with known additions at six concentrations over the range 0.1 to 425 ?g/L. Single operator precision, overall precision, and accuracy were found to be directly related to the concentration of the parameter and essentially independent of the sample matrix.

Detection Level Note:

MDLs determined per 40 CFR part 136, Appendix B.

Revision	PDF/Link
40 CFR Part 136, Appendix A (Current Edition)

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EPA-EAD: 610: Polynuclear Aromatic Hydrocarbons by GC

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