ASTM: D6592: Water Quality by Chemiluminesence

Summary
Analytes
Revision
Data and Sites

Official Method Name

Standard Test Method for Portable Chemiluminescent Water Quality Determination

Current Revision

Current edition approved March 10, 2001. Published May 2001.

Media

WATER

Instrumentation

Portable Luminometer

Method Subcategory

Organic

Method Source

ASTM

Citation

Annual Book of ASTM Standards, Section 11, Water and Environmental Technology, Volume 11.02, Water (I)

Brief Method Summary

This analytical test method is an enhanced chemiluminescent method based on the procedure of Whitehead et al. Luminol is oxidized by a complex formed between an oxidant and peroxidase (HRP) to form a luminol radical. This decomposes via an endoperoxide intermediate to form the excited state 3-aminophthalate dianion which decays to its ground state with the emission of light. The presence of enhancers results in a steady output of luminol radicals during the reaction (see Fig. 1 in the method). Contaminating substances (such as sewage, silage effluent, heavy metals, detergents, cyanides and pesticides containing organic residues) react with the assay reagents by scavenging radicals from the luminol or enhancer radicals, or by inhibiting the catalytic action of HRP. This results in a reduction of light output to a degree which is proportional to the amount of contaminant present. Light output is measured using a portable luminometer, which detects light by means of a silicon photodiode.

Scope and Application

This test method covers the detection of water and wastewater contaminants which inhibit chemiluminescence. This test method may be applied to ambient waters including but not exclusively river water and water from sewage treatment plants. It is the responsibility of the user of this standard to ensure the validity of the test method for waters of untested matrices.

Applicable Concentration Range

0-100 %

Interferences

Particulate Matter - Samples containing a high degree of particulate matter can interfere with the test. Particulate or suspended matter may be removed by centrifugation or membrane filtration if components of interest are not altered.

Quality Control Requirements

Before this test method is applied to the analysis of samples, the analyst must establish quality control by the procedures recommended in Practice D 4210 and Guide D 3856.
Quality control (QC) requirements are the initial demonstration of laboratory capability followed by regular analyses of quality control standard material. Other criteria may be more appropriate in a given situation depending on the data quality objectives.

Sample Handling

Collect all samples in accordance with Specification D 1192 and Practice D 3370, as applicable.
Samples must be collected in glass or plastic containers that are clean and flee from artifacts and or interferences. The suitability of the containers should be demonstrated for each new lot by performing a container blank.
Preserve samples by cooling to 4^oC for up to 24 h after sampling. If it is necessary to hold samples for longer than 24 hours, it is the responsibility of the user of the test to determine the maximum holding time for individual samples.

Maximum Holding Time

24 hours

Relative Cost

Less than $50

Sample Preparation Methods

This method has 1 analyte associated with it.

Analyte	Detection Level	Bias	Precision	Pct False Positive	Pct False Negative	Spiking Level
Chemiluminesence(N-12402) Chemiluminesence	N/A	101% Rec (ML)	6.50			50.00 N/A

Precision Descriptor Notes:	Nine laboratories participated in a collaborative study to determine the precision and bias of this procedure. The precision and bias values determined in this study include variability due to shipping and (where relevant) dilution of the samples used. The collaborative study and data analysis was performed using Practice D2777. Each sample was analyzed in singlicate. Since this procedure pertains to a screening test, test samples consisted of a variety of analytes in a single matrix (reagent water). The test data were obtained on reagent grade water and these precision and bias values may not be applicable to more complex matrices.
Detection Level Note:	None.

Precision Descriptor Notes:

Nine laboratories participated in a collaborative study to determine the precision and bias of this procedure. The precision and bias values determined in this study include variability due to shipping and (where relevant) dilution of the samples used. The collaborative study and data analysis was performed using Practice D2777. Each sample was analyzed in singlicate. Since this procedure pertains to a screening test, test samples consisted of a variety of analytes in a single matrix (reagent water). The test data were obtained on reagent grade water and these precision and bias values may not be applicable to more complex matrices.

Detection Level Note:

None.

Revision	PDF/Link
Current edition approved March 10, 2001. Published May 2001.

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ASTM: D6592: Water Quality by Chemiluminesence

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