IDEXX: SimPlate: SimPlate for HPC
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Official Method Name
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SimPlate for HPC, IDEXX SimPlate for HPC Multi Dose, IDEXX SimPlate for Unit Dose |
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Current Revision
| 1998 |
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Media
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WATER |
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Instrumentation
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Most Probable Number |
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Method Subcategory
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Microbiological |
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Method Source
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Citation
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Brief Method Summary
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SimPlate for HPC is intended for use in the detection and quantification of heterotrophic bacteria in water. Remove residual chlorine from water samples prior to analysis, using sodium thiosulfate. Prepare hydrated media in sterile, dechlorinated water, deionized water, phosphate buffer or 0.1 percent peptone. Pipette one ml of dechlorinated sample and 9 ml of rehydrated media onto the SimPlate; pour off the excess and incubate the SimPlate at 37 degrees C for 48 h (45 to 72 h). Read the results at 48 h by placing a 6W, 365 nm UV light within 5 inches of the sample. The heterotrophic bacteria are detected as blue-fluorescent wells on the SimPlate. Count the number of positive wells and look up the corresponding MPN value using the table provided. Reanalyze samples containing more than 738 MPN/ml, by diluting in sterile deionized water or phosphate buffer. For details, see the package insert. Requirements: SimPlate kit from IDEXX. Sterile pipets. Sterile diluent for dilutions. Incubate for 35 degrees C controlled within 0.5 degrees C. Long wavelength (365 nm) ultraviolet lamp for fluorescence readings. Refrigerator for unused, hydrated media. |
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Scope and Application
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ambient, compliance monitoring: fresh (surface & ground) & drinking water |
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Applicable Concentration Range
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for samples in wells (excluding dilution factors): 1 to 738 MPN/ml |
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Interferences
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Residual chlorine must be removed from water samples prior to analysis, using sodium thiosulfate. |
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Quality Control Requirements
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Sterility check: conducted on each lot of SimPlate received as a minimum. Use 10 ml of rehydrated media and follow directions as indicated for method. No wells should fluoresce after incubation. |
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Sample Handling
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Sample processing time: less than 5 minutes. |
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Maximum Holding Time
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Sample should be analyzed within 6 h after sampling and within 2 h from receipt of sample in lab for compliance or 24 h for routine monitoring (Standard Methods, 20th ed., Section 9060B); however, a 6 h hholding time for all samples is highly recommended (Myers and Sylvester, 1997). |
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Relative Cost
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Less than $50 |
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Sample Preparation Methods
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