Modern Water: A00074: Carbofuran in water by Immunoassay

Summary
Analytes
Revision
Data and Sites

Official Method Name

Carbofuran RaPID Assay Kit A00074

Current Revision

Revised, February 1998

Media

WATER

Instrumentation

Immunoassay

Method Subcategory

Organic

Method Source

Modern Water

Citation

Modern Water Inc. User Guides

Brief Method Summary

The Carbofuran RaPID Assay is a competitive Enzyme-Linked Immunosorbent Assay (ELISA). Carbofuran pesticide residues in the sample compete with enzyme (horseradish peroxidase)-labeled Carbofuran for a limited number of antibody binding sites on the inside surface of the test wells. The outcome is visualized with a color development step. Sample concentration is inversely proportional to color development.

Scope and Application

This method determines carbofuran in water (groundwater, surface water, well water) using immunoassay (enzyme linked immunoabsorbent assay -- ELISA).

Applicable Concentration Range

0.06 - 5 ug/L

Interferences

No interferences observed.

Quality Control Requirements

A control solution at approximately 2.0 ppb of carbofuran is provided with the Carbofuran RaPID Assay kit. It is recommended that it be included in every run and treated in the same manner as unknown samples.

Sample Handling

None specified

Maximum Holding Time

Not specified

Relative Cost

Unknown

Sample Preparation Methods

This method has 2 analytes associated with it.

Analyte	Detection Level	Bias	Precision	Pct False Positive	Pct False Negative	Spiking Level
Carbamate pesticides(E-12895) Carbamate pesticides Carbamate pesticides, N-methyl- N-methyl carbamate pesticides	N/A	N/A	N/A
Carbofuran(1563-66-2) 2,3-Dihydro-2,2-dimethyl-7-benzofuranyl methylcarbamate Carbofuran Carbofuran (ANSI) Dihydro-2,2-dimethyl-7-benzofuranyl methylcarbamate,2,3- Euradan Furadan	0.056 ug/L	N/A	N/A

Precision Descriptor Notes:	Recovery 100% at 0.5 ug/L in environmental matrices (e.g., ambient water). Precision (25 replicates over 5 days): mean within-sample at 0.44 ug/L, RSD = 6.1%; median between-sample at 0.44 ug/L RSD 10.7%. Additional data are available in the method.
Detection Level Note:	The Minimum Detectable Concentration (MDC) is based on the 90% B/Bo.

Revision	PDF/Link
Revised, February 1998

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Modern Water: A00074: Carbofuran in water by Immunoassay

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