ASTM: E-1192-97: Acute Toxicity Tests for Water with Fishes, Macroinvertebrates, and Amphibians
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Official Method Name
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Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians |
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Current Revision
| 1997 |
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Media
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WATER |
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Instrumentation
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Toxicity |
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Method Subcategory
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Acute Toxicity |
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Method Source
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Citation
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Brief Method Summary
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In each of two or more treatments, test organisms of one species are maintained for 2 to 8 days in one or more test chambers. In each of the one or more control treatments, the organsims are maintained in dilution water to which no effluent has been added in order to provide (1) a measure of the acceptability of the test by giving an indication of the quality of the test organsims and the suitability of the dilution water, test conditions, handling procedures, etc., and (2) the basis for interpreting data obtained from the other treatments. In each of the one or more other treatments, the organisms are maintained in dilution water to which a selected concentration of effluent has been added. Data on effects on the organisms in each test chamber are usually obtrained periodically during the test and analyzed to determine LC50s or EC50s for various lengths of exposure. |
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Scope and Application
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This method covers procedures for obtaining laboratory data concerning the adverse effects of an aqueous effluent on certain species of freshwater and saltwater fishes, macroinvertebrates, and amphibians usually during 2 - 4 day exposures, depending on the species, using the static, renewal, and flow-through techniques. This procedure should be useful for conducting acute toxicity tests on aqueous effluents with many other aquatic species, although modifications might be necessary. |
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Applicable Concentration Range
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Test Specific |
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Interferences
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1) Organisms should be cared for and handled properly so they are not unnecessarily stressed. 2) Toxic substances may be introduced by contaminants in dilution water, glassware, sample hardware, and testing equipment. 3) Improper effluent sampling and handling may adversely affect test results. 4) Pathogenic and/or predatory organisms in the dilution water and effluent may affect test organism survival and interfer with test results. |
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Quality Control Requirements
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Acute Test is unacceptable if one or more of the following occurred: (1) All test chambers and compartments were not identical; (2) Treatments were not randomly assigned to individual test chamber locations; (3) A dilution-water control was not included in the test; (4) The test was begun with organisms within 4 days after treatment for a disease or the organisms were treated during the test; (5) The test organisms were not impartially or randomly assigned to test chambers or compartments; (6) More than 10% of the organisms in the dilution-water control showed signs of disease or stress, such as discoloration, unusual behavior, or death, during the test; (7) Calculation of an LC50 or EC50 should usually be considered unacceptable if either or both the following occurred: 1) No treatment other than a control treatment killed or affected less than 37% of the test organism exposed to it; 2) No treatment killed or affected mor than 63% of the organisms exposed to it. |
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Sample Handling
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If sample is not used within 2h of collection, store in dark at 4 deg. C. |
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Maximum Holding Time
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N/A |
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Relative Cost
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2 - 5 days to complete test |
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Sample Preparation Methods
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In most cases sample should not be alter |
