ASTM: E1367-99: Sediment Toxicity Test Using Marine and Estuarine Amphipods
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Official Method Name
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Standard Guide for Conducting 10-day Static Sediment Toxicity Tests with Marine and Estuarine Amphipods |
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Current Revision
| Volume 11.05, August 2000. |
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Media
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SOILS/SEDIMENT |
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Instrumentation
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Organism: Refer to method for specific species and genus |
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Method Subcategory
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Acute Toxicity |
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Method Source
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Citation
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Brief Method Summary
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On the day before test initiation the sample sediment must be homogenized and placed into test chambers with overlying water added. On day 1 amphipods are randomly distributed to all test chambers for a period extending 10 days. At end of 10 day period amphipods are removed from sediment using a fine mesh sieve. Survivng amphipods are then counted. |
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Scope and Application
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This guide describes procedures for obtaining laboratory data concerning the short-term adverse effects of potentially contaminated sediment, or of a test material experimentally added to contaminated or uncontaminated sediment, on marine or estuarine infaunal amphipods during static 10-day exposures. These procedures are useful for testing the effects of various geochemical characteristics of sediments on marine and estuarine amphipods, and could be used to assess sediment toxicity to other infaunal taxa, although modifications of the procedures appropriate to the test species might be necessary. |
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Applicable Concentration Range
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Not Applicable |
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Interferences
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Physical and chemical properties of test sediment and overlying water; Endemic organisms. |
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Quality Control Requirements
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Not Applicable |
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Sample Handling
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Store samples in dark @ 4 deg. |
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Maximum Holding Time
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2 weeks |
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Relative Cost
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Greater than 5 days to complete test |
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Sample Preparation Methods
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Homogenization |
