EPA-OW: Test Method 1006.0: Larval Survival and Growth Test for Waters Using Inland Silverside
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Official Method Name
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EPA Test Method 1006.0: Inland Silverside, Menidia beryllina, Larval Survival and Growth Method. |
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Current Revision
| Third Edition, October 2002 |
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Media
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WATER |
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Instrumentation
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Toxicity |
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Method Subcategory
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Chronic Toxicity |
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Method Source
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Citation
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Brief Method Summary
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Inland Silverside, Menidia beryllina, 7 - 11 day old larvae are exposed in a static renewal system for 7 days to different concentrations of effluent or receiving water. Test results are based on the survival and growth of the larvae. |
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Scope and Application
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This method estimates the chronic toxicity of effluents and receiving waters to the inland silverside, Menidia beryllina, using seven to eleven day old larvae in a seven day, static renewal test. The effects include the synergistic, antagonistic, and additive effects of all the chemical, physical, and biological components which adversely affect the physiological and biochemical functions of the test species. |
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Applicable Concentration Range
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Effluent: 5 and a control (required) Receiving Water: 100% receiving water (or minimum of 5) and a control (recommended) |
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Interferences
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Toxic substances on glassware, hardware, etc.; Low D.O.; pH extremes; predatory organisms |
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Quality Control Requirements
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Acceptable side-by-side reference toxicant testing; 80% or greater survival in controls, with a 0.50 mg average dry weight at conclusion of test. |
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Sample Handling
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Samples must be stored at 0 - 6 deg. C prior to use Sample prep: 1. Adjust sample salinity to appropriate level. 2.Sample may need to be coarse-filtered to remove suspended solids. 3.Sample may need to be warmed slowly to test temp. 4. Dechlorination |
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Maximum Holding Time
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Sample must be used within 24h |
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Relative Cost
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Greater than 5 days to complete test |
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Sample Preparation Methods
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Above |
