EPA-ORD / EPA-OST: Test Method 100.1: 10-d Survival and Growth Test for Sediments using Hyallela azteca
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Official Method Name
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Test Method 100.1: Hyallela azteca 10-d Survival and Growth Test for Sediments |
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Current Revision
| Second Edition, March 2000 |
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Media
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SOILS/SEDIMENT |
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Instrumentation
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Toxicity |
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Method Subcategory
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Acute Toxicity |
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Method Source
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Citation
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Methods for Measuring the Toxicity and Bioaccumulation of Sediment-associated Contaminants with Freshwater Invertebrates, Second Edition, March 2000. EPA 600/R-99/064. |
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Brief Method Summary
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The day before the sediment test is initiated, each sediment should be thoroughly homogenized and added to the test chambers. At test inititation, ten 7 to 14 day old amphipods are transferred to each test chamber, and total water quality measurements are taken. 1.0ml of YCT is added to each test chamber daily (days 0-8) and temperature and dissolved oxygen are measured. Overlying water is renewed twice daily. On day 9, total water quality is measured. On day 10, temperature and dissolved oxygen are recorded and the amphipods are collected using a sieve. Survivors are then counted and prepared for weight and length measurements. |
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Scope and Application
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The objective of a sediment toxicity test is to determine whether chemicals in sediment are harmful to or are bioaccumulated by benthic organisms. The tests can be used to measure interactive toxic effects of complex chemical mixtures in sediment. Furthermore, knowledge of specific pathways of interactions among sediments and test organisms is not necessary to conduct the tests.
Sediment tests can be used to (1) determine the relationship between toxic effects and bioavailability; (2) investigate interactions among chemicals; (3) compare the sensitivitiesof different organisms; (4) determine spatial and temporal distribution of contamination; (5) evaluate dredged material; (6) measure toxicity as part of product licensing or safety testing or chemical approval; (7) rank areas of cleanup, and (8) set cleanup goals and estimate the effectiveness of remediation or management practices. |
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Applicable Concentration Range
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N/A |
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Interferences
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There are three categories of interfering factors that can cause false-negative or false positive responses: (1) those characteristics affecting survival independent of chemical concentration (Ex: UV exposure, grain size), (2) changes in chemical bioavailability as a function of sediment manipulation or storage, and (3) the presence of indigenous organisms. |
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Quality Control Requirements
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Acceptable water, test conditions, organisms. Reference toxicant testing. Performance-based criteria specifications must be met. |
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Sample Handling
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In dark at 4 deg C |
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Maximum Holding Time
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8 weeks |
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Relative Cost
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Greater than 5 days to complete test |
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Sample Preparation Methods
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Homogenization |
