Abraxis: 54002B: Benomyl and Carbendazim in water by Immunoassay, Microtiter Plate
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Official Method Name
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Abraxis Benomyl/Carbendazim ELISA Plate Kit PN 54002B |
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Current Revision
| 2007 |
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Media
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WATER |
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Instrumentation
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Immunoassay |
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Method Subcategory
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Organic |
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Method Source
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Citation
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Brief Method Summary
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Benomyl and Carbendazim are detected using a colorimetric immunoassay (ELISA) procedure. A sample (0.10 mL) and enzyme conjugate (Carbendazim-HRP) are added to plate wells pre-coated with antibodies against Benomyl and Carbendazim. Both the Benomyl/Carbendazim (if present) in the sample and Carbendazim-HRP conjugate compete to bind to the limited number of binding sites on the immobilized antibodies in proportion to their concentrations. After incubation, the unbound molecules are washed and decanted. A substrate is then added which is catalyzed by the enzyme and converted from a colorless to a blue solution. The reaction is terminated with the addition of a dilute acid. The concentration of Benomyl/Carbendazim in the sample is determined by measuring its absorbance at a specific wavelength (450nm) using a photometer and comparing its absorbance to the absorbance of the calibrators. |
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Scope and Application
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This method determines Benomyl/Carbendazim in water (groundwater, surface water, well water). |
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Applicable Concentration Range
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0.05 - 1.0 |
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Interferences
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Cross-reactivity: Benomyl and high concentrations of Thiabendazole produce false positive responses for Carbendazim. |
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Quality Control Requirements
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(A) Calibration with 3 standards and 1 blank, all analyzed in duplicate. (B) Precision: 3 matrix samples with different levels in the range for quantitative analysis analyzed daily for 5 days with 5 replicates in each of 5 assays. (C) Accuracy: 5 matrix samples spiked with the target analyte at 4 different levels in the range for quantitative analysis. (D) Validation: Analysis of 4 positive and 4 negative samples by an independent method, for confirmation. |
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Sample Handling
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Samples are collected in glass containers with Teflon-lined caps; for compliance monitoring, samples should be held no longer than 14 days. If samples are held frozen, they can be held for longer periods of time. Immunoassay reagents are stored refrigerated until use. Samples containing gross particulate matter should be filtered using a 1 micron filter or centrifuged before use. Drinking water samples are typically dechlorinated with 0.008% sodium thiosulfate. |
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Maximum Holding Time
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14 days at 4 degrees C, longer if held frozen. |
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Relative Cost
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Less than $50 |
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Sample Preparation Methods
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