Abraxis: 500090: PBDEs in water by Immunoassay, Magnetic Particle
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Official Method Name
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Abraxis PBDE Assay Kit (100T) PN 500090 |
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Current Revision
| 2005 |
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Media
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WATER |
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Instrumentation
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Immunoassay |
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Method Subcategory
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Organic |
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Method Source
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Citation
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Brief Method Summary
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PBDE is detected using a colorimetric immunoassay (ELISA) procedure. A sample (0.25 mL) and an enzyme conjugate (enzyme-labeled PBDE) are added to a disposable test tube, followed by the addition of paramagnetic particles attached with PBDE-specific antibodies. Both the PBDE in the sample and the enzyme conjugate compete for antibody binding sites on the paramagnetic particles in proportion to their concentrations. At the end of an incubation period, the magnetic particles are separated and washed with the aid of a magnetic separator. A substrate is then added which is catalyzed by the enzyme and converted from a colorless to a blue solution. The reaction is terminated with the addition of a dilute acid. The concentration of PBDE in the sample is determined by measuring its absorbance at a specific wavelength (450 nm) using a spectrophotometer, and comparing its absorbance to the absorbance of the calibrators. |
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Scope and Application
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This method determines PBDE in water (groundwater, surface water, well water, effluent) |
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Applicable Concentration Range
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20 - 1000 |
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Interferences
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Cross-reactivity: PBDE Congener 99 and PBDE Congener 28 may produce false positive responses for PBDE |
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Quality Control Requirements
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(A) Calibration with 5 standards and 1 blank, all analyzed in duplicate (B)Precision: All 46 samples analyzed in duplicate (C)Accuracy: 3 Matrix samples, spiked with the target analyte at different levels in the range for quantitative analysis (D)Validation: Analysis of 4 positive and 4 negative samples by an independent method, for confirmation |
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Sample Handling
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Samples are collected in glass containers with Teflon-lined caps; for compliance monitoring, samples should be held no longer than 14 days. If samples are held frozen, they can be held for longer periods of time. Immunoassay reagents are stored refrigerated until use. Samples containing gross particulate matter should be filtered using a 1 micron filter or centrifuged before use. Drinking water samples are typically dechlorinated with 0.008% sodium thiosulfate. |
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Maximum Holding Time
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14 days at 4C, longer if held frozen |
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Relative Cost
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Less than $50 |
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Sample Preparation Methods
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