SWAMP Bioassessment 2016

Protocol NameSWAMP Bioassessment Procedures 2016
Citation InformationOde, P.R., Fetscher, A.E., Busse, L.B., 2016, Standard Operating Procedures (SOP) for the Collection of Field Data for Bioassessments of California Wadeable Streams: Benthic Macroinvertebrates, Algae, and Physical Habitat, SWAMP-SOP-SB-2016-0001, http://www.waterboards.ca.gov/swamp.
TitleStandard Operating Procedures (SOP) for the Collection of Field Data for Bioassessments of California Wadeable Streams: Benthic Macroinvertebrates, Algae, and Physical Habitat
AuthorOde, Peter R., A. Elizabeth Fetscher, and Lilian B. Busse
Publication Year2016
Abstract

This document describes the Standard Operating Procedures (SOP) for bioassessment of wadeable streams for the California State Water Resources Control Board’s Surface Water Ambient Monitoring Program (SWAMP). These procedures are recognized by the US Environmental Protection Agency (EPA) as California’s standard bioassessment procedures and are designed to support general assessment of the ecological condition of wadeable streams and rivers based on the composition of the benthic macroinvertebrate and benthic algal assemblages. The procedures also produce standardized measurements of instream and riparian habitat and ambient water chemistry to support interpretation of the biological data.

Instructions are provided for collection of the following:
• samples for taxonomic analysis of benthic macroinvertebrate (BMI) assemblages
• samples for taxonomic analysis of benthic algal assemblages (diatoms & non-diatom (soft) algae (including cyanobacteria))
• samples for determination of biomass based on benthic chlorophyll a and benthic ash-free dry mass (AFDM)
• stream physical habitat (PHab) data
• water chemistry samples

This document represents a consolidation of two closely related previous SOPs, and supersedes them:
• Ode (2007), which focused on stream BMI sampling and associated PHab data collection and replaced previous bioassessment protocols referred to as the California Stream Bioassessment Procedure (CSBP, Harrington 1995, 1999, 2002), and
• Fetscher et al. (2009), which focused on stream benthic algae and biomass sampling, and associated PHab data collection.

Most of the methods described here are close adaptations of those developed by the EPA’s Environmental Monitoring and Assessment Program (EMAP) and currently used by the EPA’s National Rivers and Streams Assessment (NRSA) surveys. A summary of the major changes to field procedures since the previous SOPs is included.

Table of Contents

Chap. 1 Introduction
Chap. 2 Reach Delineation and Scoring Notable Field Conditions
Chap. 3 Water Chemistry Sampling
Chap. 4 Biotic Community Sampling
Chap. 5 Biotic Sample Processing
Chap. 6 Physical Habitat Transect-Based Measurements
Chap. 7 Physical Habitat Inter-Transect-Based Measurements
Chap. 8 Physical Habitat Reach-Based Measurements
Chap 9 Optional Supplemental Measures

Methods in Protocol
  • SWAMP RWB: SWAMP Reachwide Benthos (Multihabitat) Procedure